|
Light Microscopy (4 weeks)
- Theory and
Instrumentation
- Specimen
Processing for Paraffin and Cryo-sectioning
- Routine stains –
Hematoxylin and Eosin
- Special stains –
PAS, Trichromes etc
-
Immunohistochemistry including antigen retrieval methods
- In situ
hybridization – slides and whole mounts
Digital Imaging (2
weeks)
- Description of
digital images
- Image enhancement
- Image processing
- Ethics in digital
imaging
Confocal Microscopy
(3 weeks)
- Theory and
Instrumentation
- Specimen
Processing
- 2D Image
Collection and Analysis
- 3D Image
Collection and Reconstruction
- Advanced
techniques – i.e. FRET, FRAP
Live Cell Imaging (2
weeks)
- Cell Culture
Techniques
- Theory and
Instrumentation
- Short term imaging
– i.e. pH and calcium flux imaging
- Long term imaging
– i.e. cell division and migration assays
Scanning and
Transmission Electron Microscopy (4 weeks)
- Theory and
Instrumentation
- Specimen
Processing
- Operation of
Scanning EM
- Operation of
Transmission EM
Small Animal
Ultrasound Imaging (1 week)
- Theory and
Instrumentation
- Cardiovascular
Imaging
- Abdominal and
Cancer Imaging
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Laser Capture Micro-dissection and Agilent 2100 Bioanalyzer (2 weeks)
- Theory and Instrumentation
- Specimen prep and quality
control for DNA, RNA and Protein Applications
DNA Microarrays (3 weeks)
- Theory and Instrumentation
- Specimen Preparation
- Spotting and Data
Collection
- Data Analysis
Real Time PCR (2 weeks)
- Theory and Instrumentation
- Specimen Preparation
- Data collection and
analysis
Mass Spectrometry (2 weeks)
- Theory and Instrumentation
- Specimen Preparation
- Data collection and
analysis
Western and Northern
blotting (3 weeks)
- Theory and Instrumentation
(BioRad FX, Kodak IM 2000 systems)
- Specimen Preparation
- Data collection and
analysis
Flow Based Techniques (4
weeks)
- Theory and Instrumentation
(Bioplex, FACS, Sterile Cell Sorting)
- Specimen Preparation
- Data collection and
analysis
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