Fluorescence Imaging at the University of South Carolina Medical School

State of the art imaging facilities at the Medical School include a confocal MRC 1000 system along with a Voxel View Unix workstation for image analysis and reconstruction of three dimensional fluorescence images

Biorad MRC 1000 Confocal Fluorescence Microscope. Integrated Microscope Facility, University of South Carolina Medical School

Chinese hamster ovary cells transformed with wild type human transferrin receptor gene. Red shows the receptors. Green shows the endoplasmic reticulum. See Hoe, M.H. and Hunt, R.C. Journal of Biological Chemistry 267: 4916-4923, 1992

Chinese hamster ovary cells transformed with a mutant human transferrin receptor gene lacking one sugar chain. Red shows the receptors. Yellow shows the Golgi bodies stained with wheat germ agglutinin. In the picture on the right, the cells have been treated with Brefeldin A which causes Golgi body membrane to fuse with the endoplasmic reticulum. Hoe, M.H and Hunt, R.C. unpublished observations.

For quantitative fluorescence imaging the Medical School has a Meridian Instruments ACAS 570 confocal fluorescence cytometer that also has cell sorting capacities

This instrument may be used for confocal imaging

Left. Human retina stained with an anti-human haptoglobin antibody

Right: Human retina stained for transferrin receptors. From Davis, A and Hunt, R.C. J. Cellular Physiol. 156: 280-285, 1993

In both cases the fluorescence image is merged with a phase contrast image

A false color image of a confocal section through a flower bud

Fibroblasts stained with Nile red. The red color shows phospholipids in membranes and the yellow shows lipid droplets

The Meridian Instruments ACAS 570 Confocal System is available for:

Confocal imaging

Ion measurements (Ca⁺⁺, H⁺, K⁺ etc)

Fluorescence recovery after photobleaching (for molecular mobility measurements)

Kinetic studies of fluorescence redistribution

Quantitative fluorescence measurements (generation of histograms)

To use the ACAS system, please contact Dr Richard Hunt

rhunt@med.sc.edu

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This page copyright 1999, The Board of Trustees of the University of South Carolina
This page last changed on Monday, January 04, 1999
Page maintained by Richard Hunt
URL: http://www.med.sc.edu:85/image.htm
Please report any problems to rhunt@med.sc.edu