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 REAL TIME PCR TUTORIAL

SPREADSHEET FOR PFAFFL CALCULATIONS

HTML version prepared by Dr Richard Hunt and Srilata Kukunuri   Excel spread sheet for calculation of Pfaffl equation

If you get a password box, click cancel. The Excel file should then load.

The Excel file (link at logo above left) includes the formulae that we use (row 3) and also a description of what the formulae are (row 8) since the results rather than the formulae show when you print an Excel file. We do the calculation one step at a time so that it is easier to see how the calculations are done and also because we routinely like to scan for things such as whether the control gene is changing appreciably - and that is what column J in the attached file will tell you.

 
 

 

 

 

 

 

 

 

 

 

 

 

 

 

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